cd80 fitc  (Elabscience Biotechnology)

Journal: Stem cell research & therapy

Article Title: Migrasomes derived from human umbilical cord mesenchymal stem cells: a new therapeutic agent for ovalbumin-induced asthma in mice.

doi: 10.1186/s13287-025-04145-4

Figure Lengend Snippet: Fig. 3 Administration of hUCMSC-migrasomes diminished Th2 response in allergic asthma by suppressing DC maturation. A The expression of IL-4, IL-5 and IL-13 in the lung of mice in each group were detected by real-time PCR (n = 6). B Gating strategy and representative flow cytometric images for the analysis of CD45+CD4+CCR4+CXCR3− Th2 cells in the lungs of mice from each group. C Graphs showing the % of CD45+CD4+CCR4+CXCR3−Th2 cells in the lungs of mice from each group (n = 6). D Gating strategy and representative flow cytometric dot plots (left) for the analysis of CD11b+ DCs in the lungs of allergic mice and the numbers of CD11b+ DCs (right) in the lungs of mice from each group (n = 6). E The mean MFI of CD80, CD86 and MHC-II on CD11b+ DCs (n = 4–6). F The expression of IL-6 in the lung of mice were detected by real-time PCR (n = 6). G Distribution of Dir-labeled migrasomes in OVA-induced mice lung after tail vein administration (17-day injection and 18-day detected). H Representative immunofluorescence images of Dil-labeled migrasomes (red) taken up by CD11c+ cells or CD4+ cells. Data were presented as mean ± SD. A one-way analysis of variance (Tukey Kramer post hoc tests) was per formed on the data. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: For experimental group, BMDCs were pre-treated with migrasomes (20 μg/mL) for 48 h. After that, OVA (100 μg/mL) and LPS (10 ng/ mL) were added for another 48 h. After co-culturing, BMDCs cells were collected and stained for dendritic cell maturation markers, including FITC anti-mouse CD80 (Elabscience), PE anti-mouse CD86 (eBioscience) and PE anti-mouse MHC-II (eBioscience).

Techniques: Expressing, Real-time Polymerase Chain Reaction, Labeling, Injection, Immunofluorescence

Journal: Stem cell research & therapy

Article Title: Migrasomes derived from human umbilical cord mesenchymal stem cells: a new therapeutic agent for ovalbumin-induced asthma in mice.

doi: 10.1186/s13287-025-04145-4

Figure Lengend Snippet: Fig. 4 HUCMSC-migrasomes impaired DC maturation and function in vitro. A BMDCs incubated with Dil-labeled migrasomes under fluorescence micro scope (white bar = 25 μm). B The cell viability of BMDCs treated by hUCMSC-migrasomes for 24 h (left) and 48 h (right) (n = 4). C BMDCs were isolated from wild-type BALB/c mice and cultured at a density of 2 × 10⁵ cells/mL. The cells were stimulated with OVA (100 µg/mL) and LPS (10 ng/mL) for 48 h, with or without hUCMSC-migrasomes (20 µg/mL) pre-treated. After that, cells were collected for the detection of DC maturation markers including CD80, CD86 and MHC-II (n = 3). The representative images for the detection of DC maturation markers are displayed. D Statistical analysis of the mean MFIs of CD80, CD86 and MHC-II of BMDCs (n = 3). E Statistical analysis of the expression of IL-6 in BMDCs by real-time PCR (n = 3). F BMDCs (2 × 104) from WT mice were pre-treated with or without migrasomes and then pulsed with OVA323–339 and LPS and incubated at a 1:5 ratio with splenic CD4+ T cells isolated from OT-II mice (1 × 105) for 72 h. G Statistical analysis of the expression of IL-4, IL-5, IL-13 in collected cell by real-time PCR (n = 3). Data were presented as mean ± SD. A one-way analysis of variance (Tukey Kramer post hoc tests) was performed on the data. *P < 0.05, **P < 0.01, ***P < 0.001.

Article Snippet: For experimental group, BMDCs were pre-treated with migrasomes (20 μg/mL) for 48 h. After that, OVA (100 μg/mL) and LPS (10 ng/ mL) were added for another 48 h. After co-culturing, BMDCs cells were collected and stained for dendritic cell maturation markers, including FITC anti-mouse CD80 (Elabscience), PE anti-mouse CD86 (eBioscience) and PE anti-mouse MHC-II (eBioscience).

Techniques: In Vitro, Incubation, Labeling, Fluorescence, Isolation, Cell Culture, Expressing, Real-time Polymerase Chain Reaction